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KMID : 0379119840120010009
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Korean Journal of Mycology
1984 Volume.12 No. 1 p.9 ~ p.14
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Some Factors Affecting the Protoplast Release from Pleurotus ostreatus
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박용환/Park, Yong Hwan
신관철/고승주/변명옥/Shin, Gwan Chull/Go, Seung Joo/Byun, Myung Ok
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Abstract
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Some factors affecting the protoplast release from mycelia of Plurotus ostreatus using commercial lytic enzymes were investigated. The highest yields of the protoplast were obtained from four days old mycelia grown in mushroom complete medium. The solution of 0.8M MgSO₄ or KCl showed good results as the osmotic stabilizer for releasing the protoplast. Novozym 234 was the most effective among commercials tested. The concentration of the enzyme and pH of the enzyme solution were optimal at 15㎎/㎖ and 5.5∼6.0 for the protoplast release, respectively. Mycelial digestion was optimal at about 28℃ and was better in the reciprocal shaking bath (75 oscillations/min) than the stationary culture.
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